Comparison of Two ELISA Methods for the Laboratory Diagnosis of Acute Leptospirosis

نویسندگان

  • Gholamreza Abdollahpour
  • Seyyed Saeed Eshraghi
چکیده مقاله:

Background: Leptospirosis is the most common zoonosis widespread in tropical and temperate countries with low social-economic status. We aimed to compare an ELISA kit with an in-house ELISA assay to test the serum samples of the patients who were suspicious of leptospirosis according to their clinical symptoms. Methods: A total of 282 serum samples of patients suspicious of leptospirosis admitted to hospitals in Rasht city (north of Iran) were examined for sero-diagnosis of leptospirosis. Blood samples were obtained with mean time of 6.36 days after the onset of the symptoms. Antibodies were detected using a commercial qualitative and by an in-house semi quantitative IgM and IgG ELISA and the results were compared with microscopic agglutination test (MAT) as the gold standard. All specimens with titers ≥320 against a pathogenic serovar in MAT were considered positive for leptospirosis. Results: The results of MAT have demonstrated that 70 serum samples (24.8%) had a positive reaction with one of the leptospira serovar. The sensitivity, specificity, positive and negative predictive values were 87.1% , 91.0 %, 67.8%, and 95.5% for in-house ELISA assay, respectively, and 100%, 42.9%, 36.6%, and 100 % for commercial IgM ELISA assay, respectively. Conclusion: Our results showed that IgM ELISA assay is a reliable and sensitive method for the laboratory diagnosis of acute leptospirosis. In-house semi quantitative IgM ELISA was more specific and commercial IgM ELISA was more sensitive.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

comparison of two elisa methods for the laboratory diagnosis of acute leptospirosis

background: leptospirosis is the most common zoonosis widespread in tropical and temperate countries with low social-economic status. we aimed to compare an elisa kit with an in-house elisa assay to test the serum samples of the patients who were suspicious of leptospirosis according to their clinical symptoms.   methods: a total of 282 serum samples of patients suspicious of leptospirosis admi...

متن کامل

the evaluation and comparison of two esp textbooks available on the iranian market for teaching english to the students of medicine

abstract this study evaluated and compared medical terminology and english for the students of medicine (ii) as two representatives of the textbooks available on the iranian market for teaching english to the students of medicine. this research was performed on the basis of a teacher’s and a number of students’ attitudes and the students’ needs analysis for two reasons: first, to investigate...

15 صفحه اول

on the comparison of keyword and semantic-context methods of learning new vocabulary meaning

the rationale behind the present study is that particular learning strategies produce more effective results when applied together. the present study tried to investigate the efficiency of the semantic-context strategy alone with a technique called, keyword method. to clarify the point, the current study seeked to find answer to the following question: are the keyword and semantic-context metho...

15 صفحه اول

Two methods for rapid serological diagnosis of acute leptospirosis.

Leptospirosis is a common and underdiagnosed zoonosis. Two rapid assays for serological diagnosis of acute leptospirosis in diagnostic laboratories, the immunoglobulin M (IgM)-dipstick assay and the indirect hemagglutination assay (IHA), were evaluated and compared with standard assays. Sera were examined from 104 patients admitted to a hospital for investigation in a leptospirosis diagnostic p...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 35  شماره 2

صفحات  116- 121

تاریخ انتشار 2010-06-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023